This is a 5-day training workshop that will enable participants acquire the basic molecular techniques required for success in scientific research. Participants will experience hands-on training on genomic DNA extraction, electrophoresis, Polymerase Chain Reaction amplification, and RT-PCR and cDNA synthesis.

Course Objectives

The aim of this training is to equip participants with the basic knowledge and skills required to function in a molecular biology laboratory.

Date: 11-15 September 2017

Venue: Bioscience Center, International Institute of Tropical Agriculture, Oyo Road, Ibadan, Nigeria

Training language: English

Registration deadline: 31 August 2017

Course Outline

  • Laboratory safety procedures
  • The use and proper handling of Lab equipment
  • High through-put  genomic DNA Extraction
  • Nucleic Acid quantification and qualification using NanoDrop Spectrophotometer and Electrophoresis gel analysis
  • Polymerase Chain Reaction  (PCR) amplification
  • DNA Sequencing
  • RT-PCR and cDNA synthesis

Learning Outcomes

At the end of the training, participants will:

  • understand the rudiments of molecular biology and genetic research
  • be able to use some basic equipment in a molecular biology laboratory
  • be able to extract genomic DNA from plant tissues using high throughput techniques
  • be able to quantify DNA and check the purity using NanoDrop spectrometry and electrophoresis
  • understand the basic principle of PCR using molecular markers such as SSR
  • understand basic Sanger sequencing procedures be conversant with Reverse transcription PCR and cDNA synthesis

Target Audience

Research scientists, laboratory supervisors, science lecturers/teachers, technicians, graduate students and those who require knowledge of molecular biology techniques.

Faculty

Accommodation

All participants shall be responsible for their accommodation. IITA may make arrangement for interested participants on request. Accommodation request should be made at least one month before the commencement of the workshop.

Accommodation with breakfast on campus during the training period will cost about $380.

Cost

Four hundred and twenty US dollars ($420) only or Equivalent in Naira, CFA or Euro.  Fee covers Tuition, Training Materials and Refreshments.

Registration

The registration deadline is August 31, 2017. Registration is subject to confirmation of received payment. Please send payment confirmation details  and completed registration form to: IITA-TrainingUnit@cgiar.org

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Contact information

For further information contact IITA Training unit.